Fig. 3.
Northern blot analysis of hMLH1 gene transcript. Filters were hybridized with either hMLH1 probes (upper panel) or GAPDH probe (lower panel) as a control. (A) Lanes 1 through 9 are MegJ, P30OHK, BALL-1, MOLT4, MOLT16, CEM, Raji, THP1, and SKW3, respectively. (B) Lanes 1 through 12 show Ramos, Jurkat, HA, DND39, BALM1, ML1, KCL22, Ri-1, Daudi, HL60, RPMI8226, and TF1, respectively. A 2.7-kb transcript is detectable in all lanes except lane 2 of (A) and lane 7 of (B). No hMLH1 mRNA expression is found in P30OHK and KCL22.

Northern blot analysis of hMLH1 gene transcript. Filters were hybridized with either hMLH1 probes (upper panel) or GAPDH probe (lower panel) as a control. (A) Lanes 1 through 9 are MegJ, P30OHK, BALL-1, MOLT4, MOLT16, CEM, Raji, THP1, and SKW3, respectively. (B) Lanes 1 through 12 show Ramos, Jurkat, HA, DND39, BALM1, ML1, KCL22, Ri-1, Daudi, HL60, RPMI8226, and TF1, respectively. A 2.7-kb transcript is detectable in all lanes except lane 2 of (A) and lane 7 of (B). No hMLH1 mRNA expression is found in P30OHK and KCL22.

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