Fig. 6.
![Effect of HLE, Cat G, and PR3 on platelet activation induced by thrombin or TRAP42-55. Stirred fura 2-loaded platelets were preincubated with buffer (A) or 600 nmol/L of each of the neutrophil proteinases (B to D). The preincubation time was 5 minutes except for Cat G (C), for which a longer period was required to allow the Ca2+ mobilization induced by this proteinase to return to the basal value. After stimulation of platelets with 5 nmol/L thrombin, platelets were further challenged with 6.25 μmol/L TRAP42-55. Changes in fluorescence intensity reflect changes in [Ca2+]i and are representative of three distinct experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/6/10.1182_blood.v89.6.1944/3/bl_0019f6.jpeg?Expires=1720294125&Signature=KEYdjSelZcUhGMw-2WiUXH~UiKQqeNbhoKMO4KjSBMlkHFxFzvPSzep5GZhG~hZtI~SMR7wUCW1GNhefgixogYXm5XcyTO-OJ28kFcWftkvoDJSqjKbJcZwaqvWsr1cv1dXlaJJRnQp3YC54aAI61TKp0CdeWpr~4X6XXT29w8yu3Rzyf~hdVZiBrLHXL2tKYBI0JtTQcXTuSOIbXDgEwRJ-pOkA6HzoG2Q6fvHBDCcrIeu8e-aEixADhKZQQgFW6~rT--opE63PRwuAUu9-UFOoUjXV-bnOZQO1ur-YwgLcHAZBeFt3o8pdFrRfqVIe61bt-NR9pJcnnAj9jddLvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of HLE, Cat G, and PR3 on platelet activation induced by thrombin or TRAP42-55. Stirred fura 2-loaded platelets were preincubated with buffer (A) or 600 nmol/L of each of the neutrophil proteinases (B to D). The preincubation time was 5 minutes except for Cat G (C), for which a longer period was required to allow the Ca2+ mobilization induced by this proteinase to return to the basal value. After stimulation of platelets with 5 nmol/L thrombin, platelets were further challenged with 6.25 μmol/L TRAP42-55. Changes in fluorescence intensity reflect changes in [Ca2+]i and are representative of three distinct experiments.
