Fig. 4.
![Effect of HLE and Cat G on EC activation induced by thrombin or TRAP42-55. (A) [Ca2+]i was determined in fura 2-loaded IVECs in suspension preincubated for 5 minutes with 600 nmol/L Cat G or HLE before stimulation with 1 nmol/L thrombin () or 12.5 μmol/L TRAP42-55 (▨). (B) PGI2 synthesis was measured from supernatants of confluent HUVEC monolayers preincubated for 30 minutes with 50 nmol/L HLE or Cat G before stimulation with a similar concentration of the same agonists. Data are expressed as the percentage of the control values (no preincubation with HLE or Cat G). Each histogram is the mean ± SEM of three to four distinct experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/6/10.1182_blood.v89.6.1944/3/bl_0019f4.jpeg?Expires=1720291689&Signature=48Gj0wNpCtvoloB3fuapmP1wmpBWyWBtvDB4e-J3zLqE2aWXBKyKVO21deDv4fXxV7WZObnDRl-J3rOTylC1JhQfmKNbKguame1WXMXsecUi0B~jZlykRx5wcEx~tQQLFOXzFSWIk-TnyKE83VXOHPj1ZnbeMqcqaVfNE-0ovFd-TPBnKQmQGpobu~FX~hq2J3PCBnZxxA8V-33FhF6uVopb~vB8OGMjWmt21Xm6o3gT8yypcMWx3flhZ7eunZfViGZDLw6mIOvZCLcJ7kzCttE8QDn37r5eZrKiC0k-r7blLwcxUeASeJRig3NpO7WMtgJghRiy28fso0LMizBLGw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of HLE and Cat G on EC activation induced by thrombin or TRAP42-55. (A) [Ca2+]i was determined in fura 2-loaded IVECs in suspension preincubated for 5 minutes with 600 nmol/L Cat G or HLE before stimulation with 1 nmol/L thrombin () or 12.5 μmol/L TRAP42-55 (▨). (B) PGI2 synthesis was measured from supernatants of confluent HUVEC monolayers preincubated for 30 minutes with 50 nmol/L HLE or Cat G before stimulation with a similar concentration of the same agonists. Data are expressed as the percentage of the control values (no preincubation with HLE or Cat G). Each histogram is the mean ± SEM of three to four distinct experiments.
