American Society of Hematology

Fig. 1.

$Fig. 1. Fluorescence analysis of B cells derived from PB lymphocytes of a 27-year-old person (donor B; left and middle) and a 32-year-old donor (right). (Top left) Anti-IgD/anti-IgM and (bottom left) anti-IgG and IgA/anti-IgM three color stainings of CD19+ B cells enriched by MACS and the corresponding reanalyses of (top middle left) IgM++IgD−, (top middle right) IgM+IgD+, (bottom middle left) IgG+ and IgA+, and (bottom middle right) IgM++IgD+ B-cell populations. Indicated are the gates set for sorting of the respective B-cell subsets. The sorted fractions derived from this donor (B) were used for the sequence analysis of expressed Vκ3 genes and, except the IgM++IgD+ fraction, for the comparative PCR experiment. (top right) Anti-IgD/anti-IgM and (bottom right) anti-κ stainings of CD19+ B cells enriched by MACS. Indicated are the gates set for sorting of single κ+ IgM++IgD− cells.$

Fluorescence analysis of B cells derived from PB lymphocytes of a 27-year-old person (donor B; left and middle) and a 32-year-old donor (right). (Top left) Anti-IgD/anti-IgM and (bottom left) anti-IgG and IgA/anti-IgM three color stainings of CD19⁺ B cells enriched by MACS and the corresponding reanalyses of (top middle left) IgM⁺⁺IgD⁻, (top middle right) IgM⁺IgD⁺, (bottom middle left) IgG⁺ and IgA⁺, and (bottom middle right) IgM⁺⁺IgD⁺ B-cell populations. Indicated are the gates set for sorting of the respective B-cell subsets. The sorted fractions derived from this donor (B) were used for the sequence analysis of expressed V_κ3 genes and, except the IgM⁺⁺IgD⁺ fraction, for the comparative PCR experiment. (top right) Anti-IgD/anti-IgM and (bottom right) anti-κ stainings of CD19⁺ B cells enriched by MACS. Indicated are the gates set for sorting of single κ⁺ IgM⁺⁺IgD⁻ cells.

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