![Fig. 2. Dose- and time-dependent induction of Stat 1α protein and Stat1 mRNA by ATRA in NB4 cells. (A) NB4 cells (4 × 105/mL) were treated for 4 days with medium alone (medium) and medium containing the indicated concentrations of ATRA. Cells were harvested, lysed and the cell extracts (10 μg of protein/lane) subjected to Western blot analysis, using antibodies specific for Stat 1α. (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]). A Western blot representative of two independent experiments is shown. (B) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Western blot analysis was conducted as described in (A). The apparent molecular weight of standard proteins (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]) is indicated on the right. A Western blot representative of three independent experiments is shown. (C) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Total RNA was isolated and subjected (20 μg/lane) to Northern blot analysis. Filters were sequentially hybridized with cDNA probes coding for Stat 1α and β-actin. A Northern blot representative of two independent experiments is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/3/10.1182_blood.v89.3.1001/8/bl_0032f2.jpeg?Expires=1722791785&Signature=0znQnGKOUEbyafsy4kUPE94bpiiVFGoqGO~tS5adxxTVQYkEzwp~TQUaXo~tt5ht6v2q6g1RzYZe4I~GQhSZUgp6wtpctrL58RUyAOhPk-sQwqvdYFNBhXlA6m7N3CcM8rl-PIsj8N7JzMIut-fkR8se0y0DC4bKVRhfA-2dwYWSNEjc5eXMtB3Bf1kFIGOlrGe71WtjWgDmFGwWwiY10zAwut5rOQ8HBPdhuWMAyjFaj576TOjdMLp9HDKIeg8-KlVmodJ6Bbpr5uaPayx3jDPD3gzORjFqPFBTV57Rqex2dtbuWo-axVhU0u0ijQsvK1PwB9V99pWAXQqlKlijjw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dose- and time-dependent induction of Stat 1α protein and Stat1 mRNA by ATRA in NB4 cells. (A) NB4 cells (4 × 105/mL) were treated for 4 days with medium alone (medium) and medium containing the indicated concentrations of ATRA. Cells were harvested, lysed and the cell extracts (10 μg of protein/lane) subjected to Western blot analysis, using antibodies specific for Stat 1α. (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]). A Western blot representative of two independent experiments is shown. (B) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Western blot analysis was conducted as described in (A). The apparent molecular weight of standard proteins (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]) is indicated on the right. A Western blot representative of three independent experiments is shown. (C) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Total RNA was isolated and subjected (20 μg/lane) to Northern blot analysis. Filters were sequentially hybridized with cDNA probes coding for Stat 1α and β-actin. A Northern blot representative of two independent experiments is shown.
