Fig. 1.
![Fig. 1. TCR-induced IL-5 production was suppressed by glucocorticoids. A total of 105 T-cell clones ([○] HK5; [▵] YA5; [□] HK2) were cultured in 96-well round-bottom culture plates pretreated with OKT3 MoAb (10 μg/mL). Designated concentrations of hydrocortisone (A), prednisolone (B), and dexamethasone (C) were included from the start of some cultures. Culture supernatants were harvested after 24 hours and assayed for IL-5 by a specific ELISA. Data are expressed as the mean of triplicate cultures ± SEM. IL-5 production in the unstimulated cultures was always below the detection limit of the ELISA system (<1 pg/mL).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2891/4/bl_0032f1c.jpeg?Expires=1724231472&Signature=TxZL2TQHnkn3WjahJ0VOIVhsb4IYU8IeLg3yLc8JM84Wr5CiNXFqkMXoKzSsWyj1EbVyBxCUycGnxPFwtDH9yuoJUYeZ4kCOJgBu3o5wnDhJ0aek1ben0Xc~CutLyzhfCd8Ha9aeXVgSfxfbXjS-218Wi~5eNLRFhQ10tWux0e1B6QJiJxGOQvZifu964lxzOYa4CeIOulpTd6-PhbwEfNDD0WxDRIkNjVAZexUcvQbV7D68MO392vHA-qe7A2QG7cXFPjRsrFYCaGR1Mrp9BBdyV96nN2OJfNkBXnJrvTkQm5bfsE-eQpZOnxYMBxFv8n7socxVY9JRrECy4YtvWg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TCR-induced IL-5 production was suppressed by glucocorticoids. A total of 105 T-cell clones ([○] HK5; [▵] YA5; [□] HK2) were cultured in 96-well round-bottom culture plates pretreated with OKT3 MoAb (10 μg/mL). Designated concentrations of hydrocortisone (A), prednisolone (B), and dexamethasone (C) were included from the start of some cultures. Culture supernatants were harvested after 24 hours and assayed for IL-5 by a specific ELISA. Data are expressed as the mean of triplicate cultures ± SEM. IL-5 production in the unstimulated cultures was always below the detection limit of the ELISA system (<1 pg/mL).
