Fig. 4.
Fig. 4. Quantification of NGAL and MPO in cells and culture medium by ELISA. NGAL and MPO in undifferentiated (ND) and differentiated (D + RA) cells (▧) and their corresponding media (□) were assayed by ELISA. Release was calculated as the amount of the proteins in medium, given as the percentage of the total amount in medium plus cells. Mean ± SD is given. The secretion of MPO from undifferentiated cells was not significantly different from the secretion of MPO from differentiated cells (difference, 0.9% ± 8.8% [n = 18]), whereas secretion of NGAL from differentiated cells was significantly higher than that from undifferentiated cells (difference, 41% ± 8% [n = 13]).

Quantification of NGAL and MPO in cells and culture medium by ELISA. NGAL and MPO in undifferentiated (ND) and differentiated (D + RA) cells (▧) and their corresponding media (□) were assayed by ELISA. Release was calculated as the amount of the proteins in medium, given as the percentage of the total amount in medium plus cells. Mean ± SD is given. The secretion of MPO from undifferentiated cells was not significantly different from the secretion of MPO from differentiated cells (difference, 0.9% ± 8.8% [n = 18]), whereas secretion of NGAL from differentiated cells was significantly higher than that from undifferentiated cells (difference, 41% ± 8% [n = 13]).

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