Fig. 3.
![Fig. 3. Biosynthesis and sorting of NGAL in undifferentiated and differentiated transfected HL-60 cells after DFP treatment. Undifferentiated (A) and differentiated (B) transfected HL-60 cells were pulse-labeled with [35S]methionine as described in the legend of Fig 2, and DFP (final concentration, 25 mmol/L) was added 30 minutes before the end of the pulse. At indicated points, 2 × 106 cells were withdrawn and NGAL was immunoprecipitated from the cell lysates (see legend of Fig 2). The fluorograms were exposed for 48 (A) and 96 hours (B). The results of one experiment representative of two are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/6/10.1182_blood.v89.6.2113/3/bl_0025f3.jpeg?Expires=1722765822&Signature=olb6jNth7LHt3BNvYDT91MxHhOoyGk581PlxDumyN7Xfk3tSvTJvHkGXFENRXcd7OWUMY9Wdz3crfBD29eFN3QR3yWtjqdzJBSgo-y0QUNsvkqjVMiTKmK8xK-IZZR9KPlCBqR5RxY2JWp7fmIdBL0R7Kfcdc2DUx5Zs0CoQrXZpyvWVQJXRS0lNw9J9YibE13A57U2eV2XbY0-vb7WFYBNg~JFReUEfcWOG~Auy3YEKYzwz-F031u~8yG4mYvz3Sy7NlDHoULmwC3rWNZq0gFzLGtMCWAwOKbfUj4YGxHkgPSh9HUxo98FLixP5n7lyojSuCV3H8RQJdc9eqFEAPg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Biosynthesis and sorting of NGAL in undifferentiated and differentiated transfected HL-60 cells after DFP treatment. Undifferentiated (A) and differentiated (B) transfected HL-60 cells were pulse-labeled with [35S]methionine as described in the legend of Fig 2, and DFP (final concentration, 25 mmol/L) was added 30 minutes before the end of the pulse. At indicated points, 2 × 106 cells were withdrawn and NGAL was immunoprecipitated from the cell lysates (see legend of Fig 2). The fluorograms were exposed for 48 (A) and 96 hours (B). The results of one experiment representative of two are shown.
