Fig. 1.
![Fig. 1. Biosynthesis of MPO and HLA in undifferentiated and differentiated HL-60 cells. Wild-type (WT) and three clones (A, B, and C) of NGAL-transfected HL-60 cells were pulse-labeled with [35S]methionine for 3 hours, washed, and resuspended at 106 cells/mL in complete medium. Two milliliters was withdrawn for immunoprecipitation with affinity-purified rabbit antibodies against MPO or the β2-microglobulin part of the HLA complex. The fluorograms were developed for 24 hours. The positions of MPO and HLA are indicated with arrows on the right. Molecular weight markers are shown on the left.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/6/10.1182_blood.v89.6.2113/3/bl_0025f1.jpeg?Expires=1724159371&Signature=xCvj7SDQGfh29YM~KMNhVmcaK7RF2VUfZ376edTgkzHzJt1OQe6COzMwMChbiySOhEuOry3jrweDcHBt-iYeuYpnAFF8Wgb-1kKGTnKEIsOR1qY~KkFHfQ0NDKfZgJp6s01YKSJDub-D-qMqCSkSobYdHQjIwMFqN5JDnmSXAh9o31P5xSY1PfKGkIsuBVUuLOr4OeZGRSPNNEkxhWT2uYtoIq-fkIZRyO1CUk6NzTsQ1e1vC~04Dqfy2FXx0MEpZo6voPKVJ64JbRAcSg~sgv3rX22x14qQ4oXMhqQtRgAKgnfDgchs~C3agd87YpKFIYBeXlg9lPRxjm1H78m9JQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Biosynthesis of MPO and HLA in undifferentiated and differentiated HL-60 cells. Wild-type (WT) and three clones (A, B, and C) of NGAL-transfected HL-60 cells were pulse-labeled with [35S]methionine for 3 hours, washed, and resuspended at 106 cells/mL in complete medium. Two milliliters was withdrawn for immunoprecipitation with affinity-purified rabbit antibodies against MPO or the β2-microglobulin part of the HLA complex. The fluorograms were developed for 24 hours. The positions of MPO and HLA are indicated with arrows on the right. Molecular weight markers are shown on the left.
