Binding of rvWFs to collagen type III. Binding curves of WT-vWF (•), R543Q-vWF (▪), and R543W-vWF (□) to collagen type III coated microtiter wells. Incubations were performed in these wells for 2 hours at room temperature by adding increasing concentrations of rvWFs. The values shown represent the mean of a duplicate determination. The figures show a representative curve of three independent experiments (mean ± SEM).