Fig. 5.
Localization, by immunofluorescence, of CR1, band 3 antigen, and biotinylated sites on E surface. E were incubated with (A) MoAb 7G9 (anti-CR1) or (B) MoAb A9D3 (anti–band 3) followed by FITC–rabbit antimouse IgG and prepared for immunofluorescence.
(C) Biotinylated E were incubated consecutively with SA, biotinylated mouse IgG, and FITC–rabbit antimouse IgG. Parallel RIA (not shown) of E samples treated with 125I-labeled rabbit antimouse IgG (instead of the FITC conjugate) showed binding of approximately 10,000 rabbit IgG per E for all three samples.
