Fig. 1.
![Fig. 1. IL-3 regulation of apoptosis in the absence of p53. IL-3–dependent bone marrow cells from wild-type (A) or p53-null (B) mice, were plated at 2 × 105 cell/mL in a 24-well plate in the presence or absence of IL-3 after X-irradiation with 4 Gy as indicated. Cell viability was measured by trypan blue exclusion at the times shown. ([○] + IL-3; [□] + IL-3 + 4 Gy; [•] − IL-3; [▪] − IL-3 + 4 Gy.) (C) 5 × 106 wild-type or p53-null cells were analyzed for DNA fragmentation after X-irradiation as indicated with 4 Gy for 20 hours (wild-type) or 50 hours (p53-null), with or without IL-3, or after 6 hours of treatment with 10 μg/mL valinomycin in the presence of IL-3.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2717/4/bl_0010f1c.jpeg?Expires=1724145777&Signature=nv2rD~H4mrsKKAJHV31dgsFRSBV83mMUHHYG9VsA~jQ65K8TC3Pst6KTn~HiUkKit5V27LPbCjvpGbWBa42MXmO4eWSlLzuKD0pC1f~L3VbPTX9vcaf0qH7e83ea4Y5ebO14nRYURHdUqdcTPcE5G-T6g1hyb3ddXH2SgTY2pmhfgitERPeSzMGpDY7Oa9EjOZ45NAVEN9jMfaIsP1Q1sQcNvKuK8r4xgwr0rbM~npOB14kpWUYFKMIw67tYHZj3PDCrUCcmdsx~VBGeJ4JwIsjLFjTeeCEYc8CaDsmIAqgxqXaS815Lwce0SegJI3NZnH8mxbdJi6MIOQ~0mj1yRA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-3 regulation of apoptosis in the absence of p53. IL-3–dependent bone marrow cells from wild-type (A) or p53-null (B) mice, were plated at 2 × 105 cell/mL in a 24-well plate in the presence or absence of IL-3 after X-irradiation with 4 Gy as indicated. Cell viability was measured by trypan blue exclusion at the times shown. ([○] + IL-3; [□] + IL-3 + 4 Gy; [•] − IL-3; [▪] − IL-3 + 4 Gy.) (C) 5 × 106 wild-type or p53-null cells were analyzed for DNA fragmentation after X-irradiation as indicated with 4 Gy for 20 hours (wild-type) or 50 hours (p53-null), with or without IL-3, or after 6 hours of treatment with 10 μg/mL valinomycin in the presence of IL-3.
