Fig. 4.
Total cellular PKC activity in uninfected and infected erythrocytes. (A) PKC activity in 0.2 mol/L NaCl eluate from DE52 column assayed as described in Materials and Methods. Parasites were enriched to 50% by Plasmagel flotation before lysis. PKC activity is the total activity measured, minus activity in the absence of lipid activators (PMA/Phosphatidyl-serine/Triton-X–100 mixed micelles) and presence of 20 μmol/L PKC.19-36 Values represent the mean of duplicate assays + range. This result is representative of three separate experiments. (B) Non-PKC activity, as measured in the absence of lipid activators (PMA/Phosphatidyl-serine/Triton-X–100 mixed micelles) and presence of 20 μmol/L PKC,19-36 in the same RBC and IRBC lysates. Values represent the mean of duplicate values + range. This result is representative of three separate experiments.

Total cellular PKC activity in uninfected and infected erythrocytes. (A) PKC activity in 0.2 mol/L NaCl eluate from DE52 column assayed as described in Materials and Methods. Parasites were enriched to 50% by Plasmagel flotation before lysis. PKC activity is the total activity measured, minus activity in the absence of lipid activators (PMA/Phosphatidyl-serine/Triton-X–100 mixed micelles) and presence of 20 μmol/L PKC.19-36 Values represent the mean of duplicate assays + range. This result is representative of three separate experiments. (B) Non-PKC activity, as measured in the absence of lipid activators (PMA/Phosphatidyl-serine/Triton-X–100 mixed micelles) and presence of 20 μmol/L PKC,19-36 in the same RBC and IRBC lysates. Values represent the mean of duplicate values + range. This result is representative of three separate experiments.

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