Analysis of CD4 and CD34 expression on cultured CD4⁺ CD34⁺⁺ CD38⁻ Lin⁻ and CD4⁻ CD34⁺⁺ CD38⁻ Lin⁻ LDFL cells. (A) Fetal liver cells were prepared for FACS by the method described in the legend to Table 1, with the exception that streptavidin-TC was substituted for streptavidin-APC. After 4 days of culture in KL + GM-CSF + EPO, the cultured cells were restained using the same MoAbs as used for cell sorting and analyzed using a FACScan. (B) CD4⁺ CD34⁺⁺ CD38⁻ Lin⁻ and CD4⁻ CD34⁺⁺ CD38⁻ Lin⁻ LDFL cells, isolated by the identical procedure used in Table 1, were reanalyzed after 7 days of culture in KL + GM-CSF. CD34 expression was clearly observed to be greater on the cultured CD4⁺ cells than on the cultured CD4⁻ cells.