Fig. 2.
![Fig. 2. Tyrosine phosphorylation of EPR in normal and PV ECFC. Day-8 cells or FVA cells were incubated with rhEP (20 U/mL in [A] and 50 U/mL in [B]) or medium for 10 minutes (A) or 20 minutes (B) at 37°C before lysis. Human cell lysates were immunoprecipitated with anti-pTyr antibody, whereas FVA cell lysates were immunoprecipitated with antimurine EP receptor antibody. Immunoprecipitates were separated by 8% SDS-PAGE and immunoblotted with anti-pTyr antibody (A) or anti-EPR (B). The purity of the ECFC was 83% ± 2% (normal) and 65% ± 7% (PV) in (A) and 81% ± 6% (normal) in (B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/10/10.1182_blood.v89.10.3574/3/bl_0027f2b.jpeg?Expires=1723722696&Signature=SdwRj7Av4n-~zp4ThCvTEoQXvFCv2CX2z5nriuqrO67D8E6emZDuDEhfYuMarsNzjfR19FXdxVswPnMrbK9UuAvouobIVSIKUBqJJTowgvoyobAkmaynhBmjo7FUhAmcRBnYLC0ypdpJUje6LyQnFskyH9VD7pEkxmsCuJIlC-jCcqF-YSdFRQwNd8cyKZ~OKIItk6gsVt0wtUWoiNmxiO-X3mnGTcY4W~zeUnAqd6rLkuMNmxba791rNfOJky6FuIE-Ifuaud1qzwD0jOVP7UZAUiwn5ir0CgkKcnnVZ0xYU5dRc6-yVN~~mBlXseyzva~wQ~5MV~BIXX5vtmBtEQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Tyrosine phosphorylation of EPR in normal and PV ECFC. Day-8 cells or FVA cells were incubated with rhEP (20 U/mL in [A] and 50 U/mL in [B]) or medium for 10 minutes (A) or 20 minutes (B) at 37°C before lysis. Human cell lysates were immunoprecipitated with anti-pTyr antibody, whereas FVA cell lysates were immunoprecipitated with antimurine EP receptor antibody. Immunoprecipitates were separated by 8% SDS-PAGE and immunoblotted with anti-pTyr antibody (A) or anti-EPR (B). The purity of the ECFC was 83% ± 2% (normal) and 65% ± 7% (PV) in (A) and 81% ± 6% (normal) in (B).
