Lysis of HDLM-2 expressing viral proteins by CTL. Virus-specific lines and clones were generated from normal, healthy donors who shared HLA alleles with HDLM-2 by stimulating PBMC with autologous APC infected with vac recombinants. Lysis of HDLM-2 after infection with vac recombinants was determined in a standard CRA. (A) Lytic activity of a HLA-B8–restricted LMP1-specific CTL clone, 2G10 for HDLM-2 target cells infected with vac/LMP1, vac alone, or uninfected at an E:T of 2:1. (B) Lytic activity of an EBV-reactive CTL line generated from a HLA-A2,B13,18 individual for HDLM-2 target cells infected with vac/LMP1, vac/LMP2, vac alone, or uninfected at an E:T of 10:1. (C) Lytic activity of a HLA-A1–restricted HIV_gag-specific CTL clone for HDLM-2 target cells infected with vac/gag, vac alone, or uninfected at an E:T of 10:1.