Lysis of HLA-B8–positive target cells by B8-allospecific CTL clones. Polyclonal lines were generated by stimulating PBMC with allogeneic B lymphoblastoid cells derived from a homozygous A1, B8 donor. Specific lytic activity was determined in a standard CRA. Target cells include autologous LCL (negative control), LCL generated from the A1,B8 donor, allogeneic LCL matched with the stimulating LCL at only 1 class I allele, and HDLM-2. Effector cells were added at an E:T of 10:1.