FACS analysis of Class I and adhesion molecule surface expression. HDLM-2 cells were suspended in FACS media and stained with MoAbs specific for surface molecules. After addition of a second-step antibody conjugated to fluorescein, cells were analyzed by flow cytometry. (A) Negative control (▪), anti–Class I (w6/32) (), anti-CD30 (□). (B) Negative control (▪), anti-A1 (), anti-B8 (□). (C) Negative control (▪), anti-LFA2 (), anti-ICAM1 (□). (D) Negative control (▪), anti-CD80 (), anti-CD86 (□).