Agarose gel electrophoresis (a) and autoradiography (b) of Southern blot of RT-PCR products obtained from total RNA extracted from neonatal monocytes. The amplification of both 15-PGDH and iso-15-PGDH RNA(s) using 15-PGDH primers P700 and P850 from 1 μg of total RNA was performed after 1 week of culture on RNA from unstimulated (lane A) and 1,25-(OH)₂D₃ –stimulated cells (lane B).