Inhibitory effect of the addition of IL-12, anti–IL-4 MoAb or both on the development of Tc0 and Tc2 clones generated from CD8⁺ T cells of HIV-infected patients. Purified CD8⁺ T cells from PBMC of four HIV-seropositive patients were preactivated with insolubilized anti-CD3 MoAb in the absence (□) or in the presence of IL-12 (200 IU/mL) (▨), anti–IL-4 MoAb (1 μg/mL) (▨) or both (▪). After 5 days, T-cell blasts from each culture were cloned in limiting numbers, as described in Materials and Methods. Supernatants of CD8⁺ T-cell clones, obtained as described in the legend to Fig 1, were assayed for cytokine content, as described in Materials and Methods. The percentages of the four groups of clones producing IFN-γ, IL-4 and IL-5 (upper panel) and the mean values (±SE) of cytokines produced by each group of clones (lower panel) are shown.