HIV-specific cytotoxicity by CD8⁺CD30⁺ and CD8⁺CD30⁻ short-term T-cell lines generated from PBMC of HIV-infected patients. CD8⁺ T cells, stimulated with insoluble anti-CD3 Ab, were separated into CD30⁺ and CD30⁻ cells and expanded as described in Materials and Methods. IL-4 and IFN-γ production by CD8⁺CD30⁺ and CD8⁺CD30⁻ T cells was detected by cytokine intracellular staining as described in Materials and Methods (upper panels). CD8⁺CD30⁺ (•) and CD8⁺CD30⁻ (○) effector cells were incubated for 4 hours at different E:T ratios with 5 × 10⁴⁵¹Cr-labeled autologous PHA-induced CD4⁺ T-cell blasts (lower panel) (A) or EBV-transformed B-cell lines (lower panel) (B) pulsed with a mixture of eight gp120 peptides (○,•) or with medium alone (◑). The ⁵¹Cr release for each line was calculated as described in Materials and Methods. The mean values (±SE) of triplicate cultures from a representative experiment are reported.