Cytokine profile of CD8⁺ T-cell clones generated from PBMC of three HIV-seronegative and six HIV-seropositive subjects. CD8⁺ cell suspensions were preactivated with insolubilized anti-CD3 (UCTH1) Ab for 5 days and then cloned under limiting dilution conditions with irradiated autologous feeder cells, PHA and IL-2, as described in Materials and Methods. T-cell blasts from each clone (10⁶/mL) were stimulated for 36 hours with PMA and anti-CD3 (Ortho) Ab. IL-4 and IFN-γ produced in the supernatants were quantitated by appropriate immunoassays, as described in Materials and Methods. Lines represent mean values (± 5 SD) found in supernatants of stimulated cultures containing irradiated feeder cells alone.