(A) Enhanced survival/proliferation of flASK cells after transduction with the Wnt5a/LNL6 retrovirus. Transductions were initiated with 100,000 cells/mL in IL-3, IL-6, and c-kit ligand (KL). The fold expansion for LNL6- or Wnt5a/LNL6-treated cells was determined from cell counts at the end of the transduction period (48 hours) and repeated four times. (B) Suspension culture of Wnt5a/LNL6-transduced cells for 7 days results in extensive expansion compared with LNL6-treated cultures. (C) Colony formation from flASK cells after 48 hours of transduction with LNL6 or Wnt5a/LNL6. Cells were plated in quadruplicate under myeloid methylcellulose conditions using medium-containing serum, GM-CSF, G-CSF, IL-3, IL-6, erythropoietin, and KL. Colony growth was evaluated after day 12 of culture and repeated in four independent experiments.