(A through C) Cytospin analysis of flASK cells. (A) Cytospin preparations of flASK cells immediately after cell sorting or after suspension culture in (B) control CM plus 25 ng/mL KL or (C) WNT-5a CM plus KL. (D and E) Formation of adherent hematopoietic foci after cells were plated onto fibronectin-coated glass chamber slides and cultured for 4 days. Cells were fixed and stained in situ to preserve the intercellular organization of the foci. (D) Control CM plus 25 ng/mL KL. (E) WNT-5a CM (gDWnt5a) plus 25 ng/mL KL. Notice that, in (E), WNT-5a CM results in a dramatic enhancement of foci formation in which blast cells are found as adherent clusters overlying adherent cells.