(A) Human Epo gene. The five exons are shown as rectangles. Coding portions are hatched. The two fragments used in this study, Epo 5A and 3A, are shown. (B) Constructs prepared from the fragments shown in (A) and inserted into HGH-PUC12 plasmids. The coding and noncoding portions of the GH exons are shown by solid and shaded areas, respectively. (C) Diagrams of the reporter construct and the wild-type (Pwt) and the mutated GATA (Pm7) used in this study. The pEP Luc reporter construct is shown in the middle. Shown above it is the 117-bp insert from the Epo promoter. The mutation is shown as an underline. (Data from Galson et al.28 )