Fig. 7.
α4β1 -expressing neutrophils tethered to VCAM-1–transfected L cells at shears of 1.5, 1.0, and 0.5 dyne/cm2, whereas these same cells did not interact with ICAM-1–transfected L cells until very low shear stresses (A). There were approximately the same number of ICAM-1 and VCAM-1 adhesion molecules per cell (data not shown). When used alone, anti-α4 antibody (HP2/1) blocked neutrophil adhesion to VCAM-1 (B). For (A), (▪) VCAM-1 transfectants or (▵) ICAM-1 transfectants; for (B), (▪) without HP2/1 or (▵) with HP2/1. *P < .05 relative to ICAM-1 value. #P < .05 relative to neutrophils + HP2/1.
