CMRF-44 provides a convenient method for blood DC purification. (A) After in vitro culture of peripheral blood ER negative cells, density gradient separation typically enriched CMRF-44 bright DC population from <1% to 15% to 25%. (B) Double labeling with CD14 to identify the coenriched low density monocytes (70% to 80% of low density cells) allows clear separation of the CMRF-44 bright population (15% to 20% of low density cells), which can then be sorted to high purity. In this example, the CD19-PE has been added to show the small (1% to 2%) population of B lymphocytes also present. Typical sort regions are shown.