CMRF-44 labels cultured peripheral blood DC. T-lymphocyte depleted PBMC were cultured for 48 hours and labeled with the MoAb indicated. The quadrants were placed according to isotype matched control staining performed at each time point. The percentage of cells with (A) the CMRF-44 bright or (B) HB15a⁺ phenotype (shown in rectangle) was 2% to 2.5% in this individual. Antibody labeling intensity is displayed across 4 decades of log fluorescence in all figures. Results are from one of three similar experiments.