Fig. 3.
![Fig. 3. Disulfide-linked receptor complexes in the GM-CSF, IL-3 receptor family. (A) Immunoprecipitation of 125I-surface-labeled primary leukemic cells with anti-βc monoclonal antibody in the absence of ligand or in the presence of IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under nonreducing or reducing (4% 2-mercaptoethanol [2-ME]) conditions. (B) Immunoprecipitation of MO7e cells with anti-βc monoclonal antibody and immunoblotting with antiphosphotyrosine antibody. The cells were either untreated or treated with GM-CSF or IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under non-reducing or reducing (4% 2-ME) conditions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/5/10.1182_blood.v89.5.1471/3/bl_0050f3.jpeg?Expires=1723275229&Signature=UFt54L3Wc~wOIzl4gUXuTp2V-jeq5bYlWo0lMTAl2fbJeEftGlkde5jDnG9AiXZssM940lMlPv-6XnufxiVD3NOfGpiOLUcYf~KgC-k5XOPGuqBGo5wlq6zzP0Yc1xb1xmVQY5zqOpSz0WWx398lWr~dBg4u-QOKOKITLqSgdvrMAlhG2eAOhDnGUco3s1B55wfX3xVDQ-hqbxzxlI90kJeq9QSjJozA0QWWO1llOgp5TACGJG7FiRynwwjTLcI7C0pfyV~It7pb5TaFni9wHT9McLEDThHDt42UcG6P2wXOatFV8gej91Qrif5dg8Ce57cn9sixegfSU1vp79Vrbg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Disulfide-linked receptor complexes in the GM-CSF, IL-3 receptor family. (A) Immunoprecipitation of 125I-surface-labeled primary leukemic cells with anti-βc monoclonal antibody in the absence of ligand or in the presence of IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under nonreducing or reducing (4% 2-mercaptoethanol [2-ME]) conditions. (B) Immunoprecipitation of MO7e cells with anti-βc monoclonal antibody and immunoblotting with antiphosphotyrosine antibody. The cells were either untreated or treated with GM-CSF or IL-3. Proteins were separated on a 7.5% SDS-PAGE gel under non-reducing or reducing (4% 2-ME) conditions.
