IL-10 inhibits binding of NF-κB to a proximal site in the ICAM-1 promoter. Monocytes were stimulated with IFN-γ (100 U/mL), IL-10 (100 U/mL), or IFN-γ plus IL-10 in combination for 5 minutes. Nuclear extracts were analyzed for gel mobility shifts with the labeled ICAM-1 proximal NF-κB oligonucleotide. Data are representative of 3 separate experiments.