Kinetics of IFN-γ and IL-10 induction of DNA binding activity on ICAM-1 IRE. Nuclear extracts were obtained from monocytes unstimulated (control) or stimulated with IFN-γ (100 U/mL), IL-10 (100 U/mL), or the combination of IFN-γ and IL-10 and analyzed for DNA binding activity in gel mobility shifts using labeled ICAM-1 IRE. Arrows indicate binding complexes. Data are representative of 3 experiments.