Fig. 5.
![1934.4 hybridoma assay. 1934.4 hybridoma cells (5 × 104) were incubated with 5 × 105 PL/J spleen cells and various concentrations (0.01 to 1,000 μmol/L) of Ac1-11 [4A] for 24 hours in the presence of CD31 peptide (200 μg/mL) or the absence of peptide. The IL-2 production of the Ac1-11 [4A]-reactive hybridoma 1994.4 was used as a readout system for antigen presentation. IL-2 production was measured by proliferation of the IL-2–dependent cell line HT-2 in the presence of supernatants from 1994.4 cultures. Results are expressed as the mean cpm from triplicate cultures. Results are representative of 3 separate experiments](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/4/10.1182_blood.v89.4.1452/4/bl_0014f5.jpeg?Expires=1720809199&Signature=dSdXSMpE5PqU96iDouIEWlZ4~uNgS4KFzflsNAQWjGj20cjex-cGovFfF5Rf4NK2KDz2~-q0tCY3d8VrJ9SDyG95pz2EdYehVH1JYMyTdONoyLnqLV1yx1A6i9GrIAc~he4vXdf3R2rT2U7EAkzkAIHuPWJBhUZVXcYKy34I~rqR2j6LdejybU0pQouzjacDRe6dKXsET9Hxc~lqUgCxMrVApyhW3VaEPANhVdtPMhrVLRZOTBvCjBoSlsDIebHsnAtxI-N0UDjceO6~xLQujNu9D66a6JwgMI6thJL5Chjg2i~xbDrTYDDju5nAoCsQ9sg647RC4W-QeKnqe8T-wg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
1934.4 hybridoma assay. 1934.4 hybridoma cells (5 × 104) were incubated with 5 × 105 PL/J spleen cells and various concentrations (0.01 to 1,000 μmol/L) of Ac1-11 [4A] for 24 hours in the presence of CD31 peptide (200 μg/mL) or the absence of peptide. The IL-2 production of the Ac1-11 [4A]-reactive hybridoma 1994.4 was used as a readout system for antigen presentation. IL-2 production was measured by proliferation of the IL-2–dependent cell line HT-2 in the presence of supernatants from 1994.4 cultures. Results are expressed as the mean cpm from triplicate cultures. Results are representative of 3 separate experiments
