Scanning electron micrographs (SEM) of sickle cells in blood from Tg58 × Tg98 mice after deoxygenation in 1% Na₂S₂O₅ . Deoxygenated blood was fixed in buffered 2.5% glutaraldehyde and processed for SEM as described in the Materials and Methods. (A) Lower magnification to show the incidence and variety of sickle cells in deoxygenated blood. (B) Higher magnification to show more detail of the various shapes of sickled, multispiculated, and normal shaped RBCs in deoxygenated blood.