Flow cytometric analysis of murine eosinophils isolated from liver granulomas of S mansoni-infected mice. Cells were prepared as described in Materials and Methods. They were stained with fluorescein-conjugated IgE, fluorescein-conjugated anti–Fc-ε RII/CD23 (B3B4), Cy5-conjugated anti Mac-2 (M3/38), and fluorescein-conjugated anti–Fc-γ RII/RIII (2.4G2). Anti-B220 PE-conjugated and anti-Thy 1 Cy5-conjugated were used to control for B- and T-lymphocyte contamination, respectively. Gr-1 is a marker of mature granulocytes. One representative experiment of seven is shown.