(A) Expression of syndecan-1 mRNA in human HD-derived cell lines (L428, KM-H2, HDLM-2), myeloma cell lines (LP-1, U266), and myeloid leukemia cells KG-1A, as detected by RT-PCR. cDNA bulks were prepared and amplified with specific primers for human syndecan-1 (upper panel) or β-actin (lower panel). A total of 10 μL of amplified cDNAs was also run on 1.5% agarose gels, blotted onto nylon membranes, and hybridized with a syndecan-1–specific cDNA probe (center panel). (B) Western blot of cell lysates from the human myeloma cell line U266 (1.0 × 106 cells), the HD-derived cell line L428 (6.0 × 106 cells), and the myeloid leukemia cell line KG-1A (6.0 × 106 cells). The blot was incubated with 2.0 μg/mL of the MoAb B-B4 and shown by chemiluminescence. The position of the molecular weight markers is indicated.
