This RNAse protection assay demonstrates the relative proportion of processed IL-10 mRNA (201 bp) unprocessed IL-10 transcripts (197, 157, and 153 bp species) in total cellular RNA derived from the IL-10 nonproducing cell line, HTB9 (C), and from LPS-stimulated PBMNCs of preterm, term, and adult subjects. β-actin is included to assess equality of RNA loading. This is a representative blot including two of three experiments performed on cells from preterm, term, and adult subjects.