Nuclear run-on transcription assay demonstrating IL-10 transcription rate in adult (upper panel) and term neonatal blood PBMNCs (lower panel) at the time of isolation (time 0), following 3 and 6 hours in culture without LPS stimulation (control 3 hours and control 6 hours), and following stimulation with 10 μg/mL LPS (LPS 3 hours and LPS 6 hours). The IL-10 target is a 1.8-kb Xho I cDNA insert, SNURP1 is shown as an internal control, and bluescript (pBS) is shown as the negative control.