Analysis of long-term reconstitution. Progenitor populations were sorted using the parameters described in Fig 5B (box) from Ly5.2-expressing animals that had been treated with 5-FU 3 days previously. These cells were then subjected to another round of cell sorting using the same parameters to ensure the purity of the population. Either 15, 45, or 135 cells were injected into irradiated Ly5.1-expressing recipients in competition with 2 × 105 bone marrow cells from Ly5.1 donors. Mice were bled at 4, 16, and 40 weeks after reconstitution and analyzed for donor-derived cells in each lineage by staining with anti-Ly5.2 and either anti-CD3 for T cells, anti-B220 for B cells, or anti–Mac-1 or anti–Gr-1 for myeloid cells. The numbers in the table represent the percentage of donor-derived cells in each lineage for each animal at 4, 16, and 40 weeks after reconstitution.
