American Society of Hematology

Fig. 5.

Effects of the antioxidants GSH and NAC on neutrophil GSH levels (A) and Fas induced apoptosis (B). (A) Neutrophils (10 × 106/mL) were incubated for 1 hour with GSH (25 mmol/L) or NAC (25 mmol/L), washed extensively, then assayed for intracellular GSH levels as described in Materials and Methods. (B) Neutrophils (1 × 106/mL) were incubated for 1 hour with GSH (25 mmol/L) or NAC (25 mmol/L), then incubated with anti-Fas IgM (100 ng/mL) for 1 hour before washing. Apoptosis was assessed by flow cytometry 18 hours after the addition of anti-Fas IgM. *P < .05 versus control; ρP < .05 versus control PMN and medium alone. Results are the mean ± SD for n = 4 experiments with four separate donors of neutrophils.

Effects of the antioxidants GSH and NAC on neutrophil GSH levels (A) and Fas induced apoptosis (B). (A) Neutrophils (10 × 10⁶/mL) were incubated for 1 hour with GSH (25 mmol/L) or NAC (25 mmol/L), washed extensively, then assayed for intracellular GSH levels as described in Materials and Methods. (B) Neutrophils (1 × 10⁶/mL) were incubated for 1 hour with GSH (25 mmol/L) or NAC (25 mmol/L), then incubated with anti-Fas IgM (100 ng/mL) for 1 hour before washing. Apoptosis was assessed by flow cytometry 18 hours after the addition of anti-Fas IgM. *P < .05 versus control; ρP < .05 versus control PMN and medium alone. Results are the mean ± SD for n = 4 experiments with four separate donors of neutrophils.

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