Fig. 3.
Adhesion of monocytes to (4 h) IL-1β–stimulated HUVECs at a wall shear stress of 2 dynes/cm2: effects of MoAb and enzyme treatment on both firm adhesion and on monocyte transmigration, (n = 3 to 19 experiments). MoAbs CL2/6 Fab2 and Dreg-56 were used to inhibit E- and L-selectin function. MoAb HP2/1 was used to block VLA-4 on the monocyte surface while MoAb R15.7 was used against β2 integrins. Neuraminidase was used in the sialidase treatment. *Statistical significance (P < .05) compared with control with no MoAb treatments. †Statistical significance (P < .05) compared with anti–VLA-4 and anti-β2 integrin treatments.
