Spectral changes associated with apoptotic Jurkat cultures with different therapies. The 1H NMR spectra (at 400 MHz) corresponding to the Jurkat cultures before lipid extraction and TLC shown in Fig 6. (A) Control culture. The resonance peaks for choline, CH2 , and CH3 protons are shown. (B) Jurkat cells treated with 200 ng/mL of doxorubicin for 24 hours. (C) Jurkat cells deprived of serum for 3 days. (D) Jurkat cells treated with 200 ng/mL of CH 11 IgM Fas antibody for 48 hours. The CH2/CH3 signal intensity ratio, the percentages of apoptotic cells seen by nuclear morphologic analysis, and FITC-annexin V flow cytometric analysis for each culture were as follows: control (0.29, 5%, and 9.8%, respectively), doxorubicin-treated (2.55, 51%, and 47%, respectively), serum-starved (1.82, 54%, and 46.8%, respectively), and Fas antibody (1.63, 69%, and 71%, respectively).
