Time course data of doxorubicin-treated Jurkat cultures. Jurkat cultures were incubated with 200 ng/mL of doxorubicin for 0, 6, 12, 18, 24, and 48 hours. (A) The percentage of apoptotic cells in culture as observed by nuclear morphologic analysis, FITC-annexin V flow cytometry, and DNA gel electrophoresis from 0 to 48 hours after doxorubicin treatment. (B) FITC-annexin V flow cytometric data from 0 to 24 hours after doxorubicin treatment. FCS, forward scatter; FITC-annexin V, fluorescent intensity at 494 nm plotted on a logarithmic scale. (C) 1H NMR spectra obtained (with 64 excitations) at 400 MHz. The spectral resonances of choline protons (-N(CH3 )) at 3.2 ppm, methylene protons (-CH2-) at 1.3 ppm, and methyl protons (-CH3 ) at 0.9 ppm are indicated. The CH2/CH3 signal intensity ratios were 0.28, 0.56, 0.33, 0.89, 2.41, and 4.42 at 0, 6, 12, 18, 24, and 48 hours after doxorubicin treatment, respectively.
