Fig. 5.
Fig. 5. GABP activates myeloid transcription of mNE. U937 myeloid cells were transfected with 20 μg of either pNE(Δ-91)LUC (□) or pNE(Δ-79)LUC (▩), 5 μg each of pCAGGS-GABPα and pCAGGS-GABPβ or pCAGGS empty vector, and CMV/hGH (internal control). Luciferase activity was measured 14 hours after transfection and was normalized to GH expression. Data from 3 independent experiments in duplicate are shown as the mean ± SEM.

GABP activates myeloid transcription of mNE. U937 myeloid cells were transfected with 20 μg of either pNE(Δ-91)LUC (□) or pNE(Δ-79)LUC (▩), 5 μg each of pCAGGS-GABPα and pCAGGS-GABPβ or pCAGGS empty vector, and CMV/hGH (internal control). Luciferase activity was measured 14 hours after transfection and was normalized to GH expression. Data from 3 independent experiments in duplicate are shown as the mean ± SEM.

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