Fig. 6.
![Fig. 6. Gelation concentration (Csat ) of HbS and E6V(β)/D85K(α). Oxy hemoglobin samples in 50 mmol/L potassium phosphate, pH 7.5, were mixed anaerobically with dextran and sodium dithionite, incubated at 37°C, and centrifuged. The hemoglobin concentrations in the supernatant before (initial [Hb]) and after (equilibrium [Hb]) the incubation were determined by amino acid analysis. If the equilibrium [Hb] is lower than the initial [Hb], it represents the gelation concentration of Csat of the Hb.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4196/4/bl_0023f6.jpeg?Expires=1724259046&Signature=IYXlZMvkBuUm-sZigujZ~ON1Ja39igOxPTnvH50llWR6yQJJHzyObC37SyA0n4eg6YOMf4ghGQPrG512Scw0iU8-Ag3TXXneiuh5UZvD7bZwby0Ct-Rvzl0ovELYXGTbWQsg0qIB64rLbbChYoJbKziVTMj9XORr2qdkEI62EWb15f6u9YFRWSD1njnJrCwclaBXi67H01EwDwDuD-K76yzbCpObKOmgMhCbMlqNw7HN1thxaNGzzsQ8W71LfwO~wPUN37xxK4r~NimrVfHps97mp3hyM5fuAN1QQA4Vf9KsUH9KB4o-6BACwR0bkw2fcn9e6J3dbO8SjkiiGosdSA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Gelation concentration (Csat ) of HbS and E6V(β)/D85K(α). Oxy hemoglobin samples in 50 mmol/L potassium phosphate, pH 7.5, were mixed anaerobically with dextran and sodium dithionite, incubated at 37°C, and centrifuged. The hemoglobin concentrations in the supernatant before (initial [Hb]) and after (equilibrium [Hb]) the incubation were determined by amino acid analysis. If the equilibrium [Hb] is lower than the initial [Hb], it represents the gelation concentration of Csat of the Hb.
