Fig. 5.
Fig. 5. The alkaline Bohr effect of E6V(β)/D85K(α). The purified double mutant in oxy form was diluted with bis-Tris buffers of different pH values to a final concentration of 0.5 mmol/L Hb in 50 mmol/L bis-Tris, and the P50 values were determined as in Fig 4.

The alkaline Bohr effect of E6V(β)/D85K(α). The purified double mutant in oxy form was diluted with bis-Tris buffers of different pH values to a final concentration of 0.5 mmol/L Hb in 50 mmol/L bis-Tris, and the P50 values were determined as in Fig 4.

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