Fig. 2.
![Fig. 2. Time course and dose response curve of IFN-γ–stimulated AA release. HL-60 cells were prelabeled for 60 minutes with 5 μCi/mL [3H]AA, washed twice, and resuspended in the medium. After incubation with IFN-γ, cells were pelleted and the resultant supernatant was counted for 3H radioactivity. (A) Time course of AA release following treatment with 2,000 U/mL of IFN-γ. (B) Dose response of AA release at 2 minutes following IFN-γ–treatment. The data (means ± SD) are expressed as percentage of the control level obtained from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.81/2/bl_0028f2a.jpeg?Expires=1722768487&Signature=tQktJBAtgCldaxBaskNutQXLVMMqJNQTos3Pw7nzTyY9yr~uu6bngxBFMtyloba6rfkBXgKURMP3mIqbs0XHgaPzHj0CCLZY8RNu0gersWB0kaGeuUTShdcXWpXpWRqRnKTr-Ad~NnvXqYdLz493sV8NYRx2N66eeM2X-dCmgJb25lFC3YT6EfMG~NOBrUWqfCD38umpGeXlJ3~Ir5FVDjB2iZjcrBN7-sGGbrOHz1uZ1aVwyhssGj~7Mjr6JEt1YBqGMYjZQt~psQwORttFEY0e84Y6WeFIRPm7ziJUGpC8UOOf~XNy6DquYLK0bmxUnxAYYL6O8hZVPscElLWZ0w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time course and dose response curve of IFN-γ–stimulated AA release. HL-60 cells were prelabeled for 60 minutes with 5 μCi/mL [3H]AA, washed twice, and resuspended in the medium. After incubation with IFN-γ, cells were pelleted and the resultant supernatant was counted for 3H radioactivity. (A) Time course of AA release following treatment with 2,000 U/mL of IFN-γ. (B) Dose response of AA release at 2 minutes following IFN-γ–treatment. The data (means ± SD) are expressed as percentage of the control level obtained from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.
