Fig. 1.
![Fig. 1. Effect of IFN-γ on sphingomyelin (A) and ceramide (B) levels in HL-60 cells. [3H]Choline-labeled or unlabeled cells were treated at time 0 with 2,000 U/mL of IFN-γ. At the indicated times, cells were harvested, lipids were extracted, and SM levels were determined by TLC, while ceramide levels were determined using a DAG kinase assay as described in Materials and Methods. The data (means ± standard deviation [SD]) are from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/1/10.1182_blood.v89.1.81/2/bl_0028f1a.jpeg?Expires=1722768347&Signature=20Torxo5Kvdl80VIcquRTYn7it982WfspGY4D4hOZuhu3nK-PheU9cFdLt~GHdxbzBlUTuDszFfYUtm95ej3ije0Xa8yfmesQ3fQi1upfISTTA2a~5noMhO27klRxPxUDMS2oXcNv8ONBVzp0kvf-rWM5WrA-xGcFOVNqE7qGBIjTpTm4a1qq1soxxFZM2-kBe9FYFFjMgOS-vORiaVfCoFXzhWQXRP-mjKpQ0vWFMq04aIm-UiNHPrCtPsvt0Tt45VVf2o-OC1JErxav3Sl~JjFeuxKsgev6dSAIyhbaroCjob1BDgOTmHNS7nkQ0gfCPM3tkGv2w1~bMA294V3AA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of IFN-γ on sphingomyelin (A) and ceramide (B) levels in HL-60 cells. [3H]Choline-labeled or unlabeled cells were treated at time 0 with 2,000 U/mL of IFN-γ. At the indicated times, cells were harvested, lipids were extracted, and SM levels were determined by TLC, while ceramide levels were determined using a DAG kinase assay as described in Materials and Methods. The data (means ± standard deviation [SD]) are from three independent experiments assayed in duplicate. * Significantly different (P < .05, Student's t-test) from controls.
