Fig. 7.
![Fig. 7. PMN SAg-induced T-cell proliferation is HLA-DR–restricted. PMN from both an HLA-DRlow responder (donor 7; , unstimulated; □, GM-CSF/IFN-γ) and HLA-DRhigh responder (donor 2; ▨, unstimulated; ▪, GM-CSF/IFN-γ) were cultured for 44 hours in the absence or presence of 100 U/mL GM-CSF/IFN-γ, and 1 × 105 PMN from each condition were incubated with 5 × 104 CD4+ T lymphocytes in the presence of 1 nmol/L SEA. The MoAbs L243 (anti–HLA-DR), 197 (anti-CD64), and AML223 (anti-CD14) were added to the appropriate wells 30 minutes before the addition of SAg. T-cell proliferation was measured at 66 hours with an 18- to 20-hour [3H]TdR pulse and is presented as counts per minute (cpm). Values represent the mean of triplicate samples ± SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4128/4/bl_0007f7.jpeg?Expires=1724286579&Signature=kr7ki5DaduUOpjvs~Bkcoi-MBatwuwp8X3wxBPhFcJ-BEC7Ge7baIxxVqPphFh1-Mkf0EqumgW68lC-XBrO-0fWAfM33XI9JENl9oTVwQNDXCZofTwgWnMHeDQREmoSxVfFap-1r1cHPU4Mkmd30cfG3f8KTSFiJWq1Peu1uo82C4lzODPgaI-o0TABGMczyf5rzNgnsihexRrjKtUlp4nIv~PeMeNDaHAgAhU9NOQiW~AeDWcDMB~BxZLhdwzKw~6Op~XYoXYuCW3He4luHI7b4kQLjDlolORJevRcvwAw6PsqeINofCgJX5PH--NiuFnuKsxaYs6UMX3akyf8YFw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PMN SAg-induced T-cell proliferation is HLA-DR–restricted. PMN from both an HLA-DRlow responder (donor 7; , unstimulated; □, GM-CSF/IFN-γ) and HLA-DRhigh responder (donor 2; ▨, unstimulated; ▪, GM-CSF/IFN-γ) were cultured for 44 hours in the absence or presence of 100 U/mL GM-CSF/IFN-γ, and 1 × 105 PMN from each condition were incubated with 5 × 104 CD4+ T lymphocytes in the presence of 1 nmol/L SEA. The MoAbs L243 (anti–HLA-DR), 197 (anti-CD64), and AML223 (anti-CD14) were added to the appropriate wells 30 minutes before the addition of SAg. T-cell proliferation was measured at 66 hours with an 18- to 20-hour [3H]TdR pulse and is presented as counts per minute (cpm). Values represent the mean of triplicate samples ± SD.
