Fig. 3.
![Fig. 3. Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT peptide-specific T cells. Flow cytometric analysis of PMN treated with GM-CSF/IFN-γ (50 U/mL) for the presence of HLA-DR (inset) was conducted as described in Materials and Methods. The open peak represents staining with the isotype control. The closed peak represents staining with HLA-DR–specific antibody. T-cell stimulation was conducted as described in Fig 2, but using 5 μg/mL TT peptide instead of whole TT. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of triplicate samples.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4128/4/bl_0007f3.jpeg?Expires=1724286379&Signature=xhFkCLQldyZJOyihMnzCRX4ENE2l0lZNRyaAVQWQ9FsobNQAzLwrNqZsDK4VQURsiZWCoT3PjupDSeFRtZZmol7A82WBpDE~PQEMyX6Yr3rGoC6oJNymGjoF2Yk8Vb8tFWjESgQbnhN8E4S~Kmc1Ki~55fp9JGPFshV8vJtv95XCyCDaSu2zpF2EbInk1NxCsl1caLJOQ4f5wE3tR0JbbXvbBFxKM6msVtbNhkxrSo11YVOB3kBEvXREiw2C9GeNa~l7eQDv1l5C-acZcyP4hXqAx8eXfixlKFk8lyl1vPSQnSWmneje1csgtzcqNa0xZ9Do6E8Ltr7Z92UcIhFc8A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT peptide-specific T cells. Flow cytometric analysis of PMN treated with GM-CSF/IFN-γ (50 U/mL) for the presence of HLA-DR (inset) was conducted as described in Materials and Methods. The open peak represents staining with the isotype control. The closed peak represents staining with HLA-DR–specific antibody. T-cell stimulation was conducted as described in Fig 2, but using 5 μg/mL TT peptide instead of whole TT. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of triplicate samples.
