Fig. 2.
![Fig. 2. Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT-specific T cells. Purified monocytes (Mφ; 5 × 104/well) were used as control APC. PMN were first incubated for 24 hours in the presence of GM-CSF/IFN-γ (50 U/mL), and then added to wells of a 96-well plate at concentrations of 0, 1.25, or 2.5 × 105 cells/well. TT-specific T cells were than added, and the Ag presentation assay performed as described in Materials and Methods. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of duplicate samples.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/11/10.1182_blood.v89.11.4128/4/bl_0007f2.jpeg?Expires=1724287069&Signature=O1KZ36h71CX53MKe419x~zpsMTz-vm8e~lOuWknTrIqbOf1VXCqj0fJP1iS-nm80yEZyJ5ckR7vr3DKWGxdASKeRJfSPJ1VCuUF3ZACqVSdP5QO0hhhKLRHXRXs6kIOUEP7RM0K62smS~UmMNDi916EhXbaA-T1pBIHe-T4T7un6ffEpqc1F8LZMLgXPTwLhfWEh4oEIQfptmvyQyvWW23QIN1Za1RNhHiH85iwLKYZJDCyzxjmeYOh4x0g6e9S3XZuotmWOPB-OYubne9~xFGo-wRRteY6gAEkealOwtpmVnnD0Auw813Q5MMA830QEksSwnG044m51eTP6c1esXg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inability of GM-CSF/IFN-γ–treated (HLA-DR+) PMN to stimulate TT-specific T cells. Purified monocytes (Mφ; 5 × 104/well) were used as control APC. PMN were first incubated for 24 hours in the presence of GM-CSF/IFN-γ (50 U/mL), and then added to wells of a 96-well plate at concentrations of 0, 1.25, or 2.5 × 105 cells/well. TT-specific T cells were than added, and the Ag presentation assay performed as described in Materials and Methods. [3H]TdR uptake was measured at 72 to 96 hours. Data are expressed as cpm ± SD of duplicate samples.
